丙烯酰胺对胚胎脊髓神经细胞增殖分化影响

Effect of acrylamide on proliferation and differentiation of rat spinal cord nerve cell in vitro

目的研究丙烯酰胺(ACR)对胚胎脊髓神经细胞增殖分化的影响。方法利用大鼠胚胎脊髓神经细胞进行原代培养,从细胞形态学及细胞计数学角度观察不同浓度ACR对细胞生长与分化的影响;同时测定蛋白质含量、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,并与对照组进行比较。结果150,300μg/ml的ACR对大鼠胚胎脊髓神经细胞无抑制增殖和分化作用,当ACR浓度达到450,600,750μg/ml时作用才显现,并随着浓度的加大,其抑制作用增强。结论ACR能抑制胚胎脊髓神经细胞增殖和分化,可能与其能抑制蛋白质合成、DNA有关。

Objective To present experimental studies in which midbrain nerve cell of rat spinal cord were contaminated with acrylamlde（ACR）. Methods The primary culture of midbrain cell of new born rat spinal cord pruification was made after separated and digested. Acrylamide was added with doses of 0,150, 300, 450, 600, 750 μg/ml ACR respectively. The cells culture was observed with methods of cytomorghology and cell counting. The concentration rate were counted and the content of protein the MTT, malondialdehyde（MDA） and activity of superoxide dismutase（SOD） were determined. Results 150, 300 μg/ml of acrylamide had no inhibitory effect on the spinal cord neurons. The effect developed when the concentration was up to 450,600,750 μg/ml, and the inhibitory effect of acrylamide became more significant with the increase of the concentration. Conclusion The inhibition of acrylamide on rat spinal Cord nerve cells might be associated with impact of protein synthetis and DNA.