纳米银仿生敷料研制及使用效果评价

Development and effect evaluation on nano-silver bionic wound dressing

目的研制纳米银仿生敷料并观察治疗SD大鼠深Ⅱ度切割伤的效果及其安全性。方法将42只清洁级SD大鼠用取皮刀在其背部造成10％～13％总体表面积的深Ⅱ度切割伤，并随机分为3组：纳米银仿生敷料组、磺胺嘧啶银暴露保痂组及壳聚糖膜组，观察各组愈合情况。并于术后2，4，5，7，13，16d眼眶采虹，测定各组虹银的含量。术后45d处死鼠，取肝、脑、肾组织消化测定银含量。结果自制纳米银仿生敷料组术后10d和13d愈合率分别为88．50％和98．98％，明显高于其他2组，差异有统计学意义（P〈0．01）。纳米银仿生敷料组大鼠不同组织中银含量均低于磺胺嘧啶银对照组，差异有统计学意义（P〈0．01），且该组中血银含量较磺胺嘧啶银暴露保痴组较快恢复至正常水平。结论自制纳米银仿生敷料具有促进创面愈合的作用，且与磺胺嘧啶银比较，减少了银中毒的可能性，为该敷料进一步应用于临床提供了安全性依据。

Objective To investigate the efficacy of nano - silver bionic wound dressing. Methods Sprague Dawley rats （SD rats） were randomly divided into three groups： nano- silver dressing group （ n = 14）, sulfadiazine silver （SD - Ag） group （n = 14） and chitosan film group （n = 14）, and then deep partial- thickness wound involving 10-13% total body surface area （TBSA） were reproduced in the three groups. Blood samples on post - operation day 2, 4, 5, 7, 13 and 16 and liver, brain and kidney on post - operation day 45 were harvested for the determination of trace silver. Results The wound healing rate was higher in nano - silver dressing group than that in other two groups （ P 〈 0.01 ）. The silver level in tissues and blood was lower than SD-Ag group （P 〈 0.01）. The blood silver content in nano- silver group returning to normal level was faster than that in SD - Ag group. Conclusion The nano- silver wound dressing can promote the healing of wound, and it can reduce the possibility of silver poisoning. Its good efficacy and safety shows a prosperous future in clinical setting.