连翘提取物对小鼠T淋巴细胞体外活化与增殖的影响

Effects of Forsythia suspense extract on cell proliferation and activation of the mouse T lymphocytes in vitro

目的:分析连翘(FS)提取物对小鼠淋巴结T细胞的体外活化与增殖的影响,初步探讨其免疫抑制作用机制。方法:无菌分离小鼠淋巴结细胞,加入多克隆刺激剂刀豆蛋白A(ConA)进行刺激,利用荧光标记的单克隆抗体(mAb)染色结合流式细胞术(FCM),检测小鼠T淋巴细胞的表达的活化抗原CD69、CD25、CD71的表达情况;以羧基荧光素乙酰乙酸琥珀酰亚胺酯(CFDA-SE)染色,以FCM分析FS对淋巴细胞体外增殖的影响。结果:终浓度为40、80、160 mg/L的FS均对ConA刺激诱导的T细胞CD69、CD25和CD71的表达有降低作用(P<0.05)。CFDA-SE染色分析显示,上述浓度的FS对ConA诱导的小鼠T淋巴细胞增殖具有抑制作用(P<0.05)。结论:FS对ConA诱导的T细胞早、中、后期活化和体外增殖有抑制作用。

AIM： To investigate the effect of Forsythia suspensa （FS） extract on the activation and proliferation of the mouse T lymphocytes in vitro as well as its immunosuppressive effect. METHODS： The lymphocytes were isolated from the lymphoid nodes of BALB/c mice. Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69, CD25 and CD 71 of the activated T lymphocytes in vitro in response to Concanavalin A （ConA）. Stained with CFDA-SE, the mouse lymphocytes were stimulated by ConA. The effect of FS extract on cell proliferation in vitro was analyzed by flow cytometry. RESULTS： FS extract decreased the expression of CD69, CD25 and CD71 at the final concentration of 40, 80 and 160 mg/L, respectively（ P 〈 0. 05 ）. CFDA-SE staining showed that FS extract at different concentration significantly inhibited the proliferation of lymphocytes induced by ConA （P〈0.05）. CONCLUSION： FS extract can inhibit the activation and proliferation of T lymphocytes in response to ConA.