摘要
目的:分析连翘(FS)提取物对小鼠淋巴结T细胞的体外活化与增殖的影响,初步探讨其免疫抑制作用机制。方法:无菌分离小鼠淋巴结细胞,加入多克隆刺激剂刀豆蛋白A(ConA)进行刺激,利用荧光标记的单克隆抗体(mAb)染色结合流式细胞术(FCM),检测小鼠T淋巴细胞的表达的活化抗原CD69、CD25、CD71的表达情况;以羧基荧光素乙酰乙酸琥珀酰亚胺酯(CFDA-SE)染色,以FCM分析FS对淋巴细胞体外增殖的影响。结果:终浓度为40、80、160 mg/L的FS均对ConA刺激诱导的T细胞CD69、CD25和CD71的表达有降低作用(P<0.05)。CFDA-SE染色分析显示,上述浓度的FS对ConA诱导的小鼠T淋巴细胞增殖具有抑制作用(P<0.05)。结论:FS对ConA诱导的T细胞早、中、后期活化和体外增殖有抑制作用。
AIM: To investigate the effect of Forsythia suspensa (FS) extract on the activation and proliferation of the mouse T lymphocytes in vitro as well as its immunosuppressive effect. METHODS: The lymphocytes were isolated from the lymphoid nodes of BALB/c mice. Fluorescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69, CD25 and CD 71 of the activated T lymphocytes in vitro in response to Concanavalin A (ConA). Stained with CFDA-SE, the mouse lymphocytes were stimulated by ConA. The effect of FS extract on cell proliferation in vitro was analyzed by flow cytometry. RESULTS: FS extract decreased the expression of CD69, CD25 and CD71 at the final concentration of 40, 80 and 160 mg/L, respectively( P 〈 0. 05 ). CFDA-SE staining showed that FS extract at different concentration significantly inhibited the proliferation of lymphocytes induced by ConA (P〈0.05). CONCLUSION: FS extract can inhibit the activation and proliferation of T lymphocytes in response to ConA.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2008年第1期10-12,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家重点基础研究发展计划(973)资助(2006CB504200)
广州市科技局科技攻关重点资助项目(2006Z-E0091)
关键词
连翘提取物
T淋巴细胞
活化
增殖
Forsythia suspensa extract
T lymphocytes
activation
proliferation