CYP17基因多态性与乙型肝炎病毒感染后肝硬化、肝细胞癌的关系

Genetic polymorphisms of CYP17 and risks of cirrhosis or hepatocellular carcinoma in hepatitis B-infected patients

目的研究参与雌激素代谢的细胞色素P45017(Cytochrome P45017,CYP17)MspA1I位点基因多态性与山东地区汉族人乙型肝炎病毒(hepatitis B virus,HBV)感染后肝硬化、肝细胞癌(HCC)的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对山东地区96例乙肝相关性HCC患者、107例乙肝后肝硬化患者、82例慢性乙型肝炎患者、91例乙型肝炎表面抗原(HBsAg)携带者、80例健康对照组的CYP17基因MspA1I位点的T/C多态进行研究。结果与携带者相比,CYP17 A2/A2基因型明显增加了女性HBV感染患者HCC的发病风险(OR=5.93,P=0.002),尤其在绝经后的妇女,OR增加至6.18,P=0.003,而在男性患者中差异无统计学意义。乙肝肝硬化患者与携带者相比,A2/A2基因型频率差异亦无统计学意义(P>0.05)。结论CYP17 MspA1I多态位点A2/A2基因型与山东地区女性HBV感染后HCC的易感性有关。

Objective To explore the relationship between genetic polymorphisms of CYP17 MspA1I and cirrhosis or hepatocellular carcinoma in hepatitis B infected patients of Shandong Pmvince. Methods 80 healthy controls and 376 HBV patients including 96 hepatocelluar carcinomas（HCC）, 107 cirrhosis, 82 hepatitis and 91 HBsAg carriers were determined the T→C in CYP17 MspA1I by palymerase chain reaction and restriction fragment length palymorphism （PCR-RFLP）. Results Compared with the carriers, the （ - 34） A2/A2 genotype of CYP17 significantly increased the risks of HCC patients. Females mostly contributed to this association （ OR = 5.93, P = 0.002）, and the OR values increased in post-menopausal women （ OR = 6.18, P = 0.003） but not in male subjects. There were no significant differences in the distribution of CYP 17 genotypes between carriers and cirrhosis （ P 〉 0.05）. Conclusion CYP17 MspA1I genotypes A2/A2 may be related to liver cancer risk in HBV-infected women in Shandong Province.