摘要
目的探讨同种异体兔骨髓间充质干细胞（marrow mensenchymal stem cells，MSCs）与纳米晶胶原基骨（nano—hydroxyapatite／collagen，NHAC）修复材料构建的组织工程骨修复兔胫骨缺损的可行性。方法24只新西兰大白兔胫骨中段形成10mm长的骨缺损，右侧骨缺损处植入组织工程骨作为实验组，左侧骨缺损处植入单纯NHAC作为对照组。术后3、6、9、12wk分批处死动物，行一般情况、缺损区大体观察、X线、组织学染色分析等指标检测，行统计学分析，比较各组修复骨缺损的效果。结果24只新西兰大白兔均进入结果分析。①术后一般情况：各组兔术后恢复及进食均正常，伤口无炎症反应，愈合良好。②大体观察：实验组术后6wk骨缺损部分修复，9、12wk骨缺损完全修复，3、6、9、12wk骨缺损修复情况明显好于对照组。③X线：实验组缺损区术后3wk可见有骨痂生长，9wk骨缺损基本修复，对照组术后12wk缺损区基本修复，各观察时间点实验组骨缺损修复情况明显好于对照组。④组织学染色：实验组缺损区新生类骨样组织、编织骨和板状骨出现的时间较对照组早，并且不经软骨介导即可直接成骨，而对照组以“爬行替代”方式修复骨缺损。结论同种异体兔MSCs复合NHAC修复骨缺损的能力较单纯NHAC强且迅速，能够对大段骨缺损进行快速有效的修复。
Objective To explore the feasibility of tissue - engineering bone, constructed with allogenic bone marrow mensencbymal stem cells （ MSCs ） and nano - hydroxyapatite/collagen（ NHAC）, in repairing of rabbit tibial long bone - periosteum defects. Methods 24 healthy adult New Zealand white rabbits made the model of 10mm bone - periosteum defects in the middle of bilateral tibias, Rabbits with tissue - engineering bone planted on the right side as the experimental group and NItAC planted on the left side as the control group, General conditions, macroscopic evaluation, X -ray and histomorphology examination of bone defect repairing sample and statistical analysis were performed 3.6. 9. 12 weeks postoperatively in order to compare the effects of repairing bone defects of three groups. Results All the 24 rabbits entered the stage of final analysis. （ 1 ） Postoperative general conditions＇the recovery and diet in both groups were normal, and the incisions healed well without inflammatory reactions. （2）Macroscopic evaluation： the bone defects of the experimental group were partly repaired 6 weeks after operation and completely repaired 9 or 12 weeks after operation. , and the repair of bone defects of experimental group were better than it of control group. （ 3 ） X - ray examination： in the experimental group, the formation of scars can be seen at postoperative 3 weeks, and the bone defects were generally repaired at postoperative 9 weeks; in the control group, the bone defects were generally repaired at postoperative 12 weeks; the repair of bone defects of experimental group were better than it of control group at each of the 4 observing times. （4） histomorphology： in the experimental group, the osteoid tissue, woven bone and lamellar bone occurred earlier than it in the control group, and the new bone formed directly with- out through a cartilaginous intermediate, while the defect was repaired in a ＂ creep substitution＂ way in the control grouP. Conclusion The bone defect repair ability of tissueengineering bone which constructed with allogenic MSCs and NHAC is strong compared with NHAC alone, so the tissue - engineering bone could repair the long bone defects rapidly and effectively.
基金
江苏大学临床医学科技发展基金项目资助（JLY20050008）
