基因打靶突变小鼠的早期T细胞发育

Early T Cell Development in Gene Targeted Mutant Mice

早期Ｔ细胞的发育是一个受到多种分子精确调控的过程，基因打靶技术的建立和发展为体内研究上述分子的作用提供了有效的手段。对ＴＣＲ、ＣＤ３基因打靶小鼠的研究表明，ＣＤ４４－ＣＤ２５＋阶段是早期Ｔ细胞发育的重要调控点，在此发育阶段，由ＴＣＲβ、ＴＣＲα和ＣＤ３成分组成的ｐｒｅ－ＴＣＲα复合体的表达或其与未知配体的结合通过ｐ５６ｌｃｋ传递信号，介导ＣＤ４４－ＣＤ２５＋细胞的进一步发育，该复合体任何成分的缺失都将使Ｔ细胞发育不能正常进行。

Early intrathymic T cell development is highly coordinated and controlled by the interaction of a variety of molecules. The development of gene targeting technology provided a valuable tool to study the function of these molecules in vivo. The analysis of TCR and CD3 gene targeted mice indicated that CD44 -CD25 + is a control point in early T cell development. At this stage, the expression of pre TCR complex (composed of pre TCRα,TCRβ and CD3) and/or its combination with unknown ligand provide signals for further progression beyond the CD44 -CD25 + stage. The deficiency of any component in pre TCR complex would lead to the blockage of T cell development at early stages.