垂体腺苷酸环化酶激活肽对血管壁细胞成分的作用及其机制的实验研究——PACAP与动脉粥样硬化关系的探讨

Experimental Study of the Effects of Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) and Its Mechanism on the Vascular Cell Components——The Possible Relationship between PACAP and Atherosclerosis

以培养的猪主动脉内皮细胞（ＥＣ）和肺动脉平滑肌细胞（ＳＭＣ）为主要实验对象，研究了垂体腺苷酸环化酶激活肽（ＰＡＣＡＰ）对正常及高脂环境下培养的ＥＣ、ＳＭＣ形态和功能的影响，并对其作用机制进行了初步探讨。结果显示：ＰＡＣＡＰ可部分对抗高脂因素造成的ＥＣ、ＳＭＣ形态的损伤；能提高ＥＣ抗动脉粥样硬化（ＡＳ）物质的产生；抑制ＳＭＣ的增殖；并具有抗脂质过氧化作用。本研究表明，ＰＡＣＡＰ对ＥＣ、ＳＭＣ具有一定程度的细胞保护作用，因此提示ＰＡＣＡＰ可能具有一定的抗ＡＳ作用。

By using cultured vascular endothelial cell (EC) and smooth muscle cell (SMC) as model, the morphological and functional effects of PACAP on EC and SMC in normal and hyper lipid culture circumstances as well as its possible mechanism of action were studied. The results showed that (1) PACAP may partly counteract the morphologic injury of EC and SMC produced by high lipid. (2) PACAP significantly increased the production of anti AS substances by EC. (3) PACAP inhibited the proliferation of SMC. (4) PACAP reduced significantly the production of lipid peroxide by EC and SMC in hyperlipid circumstances. The present study suggests that PACAP has cytoprotective effect on EC and SMC and thus it is possible that PACAP has an anti AS effects.