三七总皂苷对急性坏死性胰腺炎大鼠肺组织核转录因子-κB活性及肺损伤的影响

Effects of panax notoginseng saponins(三七总皂苷)on activity of lung nuclear factor-κB and acute lung injury in rats with acute necrotizing pancreatitis

目的探讨三七总皂苷能否抑制急性坏死性胰腺炎（ANP）大鼠肺核转录因子-kB（NF-kB）的活性，并减轻ANP时肺组织病理损害。方法30只SD大鼠被随机分为假手术组（sham组）、ANP模型组和三七总皂苷预处理组，每组10只。sham组和ANP模型组制模前1h腹腔注射质量分数为0．9％的生理盐水（1ml／kg），三七总皂苷组制模前1h腹腔注射50g／L三七总皂苷（1ml／kg）。用质量分数为5%的牛磺胆酸钠经大鼠胰胆管逆行注射制备大鼠ANP模型；sham组进腹后仅翻动胰腺和十二指肠后关腹，不注入牛磺胆酸钠。各组于制模6h后处死大鼠，取肺组织测定湿／干重（W／D）比值和NF-kB活性。结果ANP模型组肺组织NF-kB阳性细胞率70％～100％所占比例（7只比0只）和阳性细胞数[（72．52±7．63）PU比（33．09±4．75）PU]明显高于sham组（P均〈0．05），经三七总皂苷预处理后NF-kB阳性细胞率70％～100%所占比例（0只比7只）和阳性细胞数[（42．77±9．79）PU比（72．52±7．63）PU]较ANP模型组明显下降（P均〈0．05）。ANP模型组肺组织病理评分及W／D比值均较sham组显著增高，而三七总皂苷组较ANP模型组明显改善（P〈0．05或P〈0．01）。结论NF-kB在ANP大鼠肺组织中被明显激活；三七总皂苷可以在体内抑制肺组织中NF-kB的激活，减轻各脏器的病理损害。

Objective To investigate whether panax notoginseng saponins （三七总皂苷,PNS） can inhibit the activity of lung nuclear factor-kB （NF-kB） and ameliorate the lung pathologic injury in rats with acute necrotizing pancreatitis （ANP）. Methods Thirty Sprague-Dawley （SD） rats were randomly divided into three groups： sham operation group （group sham）, ANP group （group ANP）, PNS preconditioning group （group PNS）. There were 10 rats in each group. Rat ANP model was induced by retrograde injection of 5 sodium taurocholate to pancreatic duct （1 ml/kg）. The rats in the PNS preconditioning group were given by intraperitoneal injection of 50 g/L PNS （1 ml/kg） one hour before model establishment, and those in the other two groups were given 0.9% physiological saline （1 ml/kg） one hour before the operation. Put the rat in each group to death at 6 hours after the respective management was made, and the pulmonary tissues were taken to detect the activity of NF-kB by immunohistochemistry and the wet/dry weight ratio of the lung tissue was measured. Results At 6 hours after the model was established in the ANP group, the NF-kB positive cell number （70%-100% positive cells, 7 vs. 0） and the positive unit [（72.52±7.63）PU vs. （33.09±4. 75）PU] in the lung tissue were remarkably increased in comparisons with those in the sham group （both P〈0. 05）. After PNS preconditioning, the NF-kB positive cell number （70%- 100% positive cells,0 vs. 7） and the positive unit [（42.77±9.79） PU vs. （72.52±7.63） PU, both P〈0.05]were decreased significantly in comparisons with those in the ANP group. Histopathology scores and wet/dry weight ratio of lung in group ANP were significantly increased compared with group sham, while those were improved in group PNS compared with group ANP （P〈0.05 or P〈0.01）. Conclusion NF-kB is activated obviously in the lung tissue in ANP rat; PNS may inhibit the activation of NF-kB in the lung tissue in the body and ameliorate the pathological injury in the various viscera.