响应面法优化链霉菌702产抑真菌物质生料发酵培养基

Optimization of Culture Medium for Anti-fungus Compound Produced by Streptomyces 702 by Means of Response Surface Method Under Unsterilized Condition

用响应面分析方法对链霉菌702产抑真菌物质生料发酵培养基进行了研究。实验首先利用部分重复因子试验筛选出链霉菌702发酵产抑真菌物质的关键因素为玉米淀粉和蛋白胨,其次利用最陡爬坡试验逼近最大响应区,最后利用中心组合设计及响应曲面分析确定关键因素的最佳浓度。通过优化得到玉米淀粉和蛋白胨的最适浓度分别为16.83 g/L、8.64 g/L,在优化后的培养基中链霉菌702产抑真菌物质效价达到1 214.52μg/mL,比优化前789.41μg/mL提高了53.85%。

The response surface method was used to optimize the culture medium for anti -fungus compound produced by Streptornyces 702 under unsterilized condition. In the first optimization step cornstarch and peptone were screened out,which were the critical factors affecting anti -fungus compound produced by Streptomyces 702 using Fractional Factorial Design. Then the steepst ascent path was used to approach the optimal region of the medium composition. In the third step the optimal concentration of cornstarch and peptone were determined by a central composite design and response surface analysis. The optimum concentration of cornstarch and peptone were 16.83g/L,8.64g/L, under the optimum medium the anti - fungus compound production was increased from 789.41 μg/mL to 1214. 52μg/mL by 53.85%.