毛细管电泳安培法分离测定安痛定注射液中安替比林和氨基比林的含量

Determination of Antipyrinum and Amidopyrine by Capillary Electrophoresis with Amperometric Detection

采用自行设计的毛细管电泳电化学检测系统,对安痛定注射液中2组分安替比林和氨基比林进行了分离检测。在pH=9.0的30mmol/L硼砂缓冲溶液中,分离高压为20kV,进样时间为8s,检测电位为0.8V的条件下,安替比林的线性范围为0.5～100mg/L,线性系数为r=0.9992,检出限为0.01mg/L;氨基比林的线性范围为2～160mg/L,线性系数为r=0.9985,检出限为0.05mg/L。

On the self-constructed electrochemical detection system, antipyrinum and amidopyrine in injection antondint were separated and determined by capillary electrophoresis with amperometric detection （CE-AD）. Under the optimized conditions： a running buffer composed of 30 mmol/L Na2B4O7 buffer pH 9. 0, separation voltage 20 kV, injection time 8 seconds and detection voltage 0. 8 V, a linear relationaships between peak areas（Y） and mass concentrations（c） of the two analytes were established, with a determination limit of 0. 1 mg/L for antipyrinum and that of 0. 05 mg/L for amidopyrine, respectively.