膜型-Ⅰ型基质金属蛋白酶mRNA在人喉鳞癌组织中的表达

Expression of membrane type-Ⅰmatrix metalloproteinase-mRNA in human laryngeal squamous cell carcinoma

目的:为研究人喉鳞状细胞癌在其浸润转移中的的生物学行为,探讨能导致肿瘤浸润转移的膜型-Ⅰ型基质金属蛋白酶mRNA在人喉鳞癌组织不同部位的定位及表达情况。方法:采用原位杂交方法对人喉鳞癌组织的原发灶50例、癌旁组织34例、淋巴结22例、正常粘膜20例进行膜型-Ⅰ型基质金属蛋白酶mR-NA的进行检测,图像分析做定量分析。结果:原发灶中MT1-MMP mRNA主要在肿瘤细胞的胞浆中呈强阳性表达,进展期肿瘤癌巢周边的基质细胞中呈弱阳性表达。在癌旁、转移淋巴结的肿瘤细胞胞浆中有阳性表达。原发灶中MT1-MMP mRNA表达与癌旁、正常粘膜组织均有显著差异(P=0.000);与淋巴结之间存在显著差异(P=0.004);正常粘膜与淋巴结之间也存在显著差异(P=0.000);癌旁与淋巴结之间无显著差异(P=0.900);癌旁与正常组织之间亦无显著差异(P=0.260)。结论:MT1-MMP mRNA在原发灶、癌旁(淋巴结)、正常组织中的表达呈递减趋势。提示其在喉癌浸润转移的生物学行为中有重要作用。

Objective：To study human laryngeal carcinoma biology behavior in invasion and metastasis with the expression of membrane type I matrix metalloproteinase （MT1 -MMP） . Methods： In 50 laryngeal carcinoma patients, the 50 samples of tumor, 30 samples of transitional tissue, 22 samples lymph nodes and 20 samples of distant normal tissues were tested . The localization of MT1 -MMP and MMP -2 were determined by in situ hybridization, and their expression quantity was assayed by image analysis system. Results ： MT1 - MMP mRNA was localized predominantly in carcinoma cells cytoplasms with relatively weak stained in the stromal cells around carcinoma nest, these positive expressions of MT1 - MMP mRNA occurred in the carcinoma cell cytoplasms of the transitional tissues and metastasis lymph node too. MT1 - MMP mRNA expressions in primary tumors were different with transitional tissues, lymph node and normal tissues （P 〈0. 05）. It was different between normal tissues and lymph nodes （ P = 0.00）. It was not different between transitional tissues and lymph nodes （P = 0.90）, not different between transitional tissues and normal tissues too （P = 0. 260）. Conclusion ： MT1 - MMP mRNA expression in primary, transitional （ lymph node） and normal tissues are decreased. It plays a role in the biological behavior of laryngeal carcinoma invasion and metastasis.