卵泡刺激素受体在成年化疗大鼠卵巢中的表达

In vivo expression of FSHR in rats ovaries after exposure to cyclophosphamide

目的:探讨化疗药物所致的卵巢病理损伤以及FSHR在化疗卵巢中的表达.方法:将成年大鼠随机分为两组:生理盐水组和环磷酰胺组.大鼠单次腹腔注射环磷酰胺,观察卵巢形态学改变并计数卵泡数目.荧光定量PCR方法检测大鼠卵巢FSHR mRNA的表达.结果:环磷酰胺可引起原始卵泡大量丢失,卵巢间质严重损害,且卵巢局部发生纤维化.化疗组卵巢窦卵泡比例显著低于对照组(P<0.05);化疗组大鼠动情周期日数[(6.83±1.12)d]显著高于对照组[(4.50±0.82)d,P<0.05].荧光定量PCR显示,环磷酰胺组FSHR mRNA水平显著低于对照组(P<0.05).结论:化疗大鼠卵巢FSHR表达减少,可能是导致化疗卵巢局部窦卵泡比例减少和动情周期延长的原因之一.

AIM： To examine the pathological changes of ovaries and the effects of the expression of mRNA transcripts of FSH receptor in ovaries of the rats exposed to chemotherapeutic drugs. METHODS： Twenty rats were randomly assigned into two groups： cyclophosphamide group and normal saline group, who received a single injection of the saline or chemotherapeutic agent cyclophosphamide. Animals were sacrificed after 8 weeks of treatment. Ovarian pathological changes were observed and follicle number was counted. FSH mRNA was quantified using real-time PCR. RESULTS： Cyclophosphamide had a detrimental effect on ovarian stromal function. The rats in cyclophosphamide group developed a lower proportion of antral follicles compared with control group（ P 〈 0.05 ）. FSHR mRNA content was significantly lower in the cyclophosphamide treatment group than in the control group （P 〈 0.05 ）. There was a significant positive correlation between FSHR and antral proportion and a significant negative correlation between FSHR and duration for estrous cycle （P 〈 0. 05 ）. CONCLUSION： In ovaries exposed to cyclophosphamide lower, there is lower level of FSHR, which may be involved in antral follicle development blockade and may be associated with prolonged estrous cycle.