摘要
目的:应用蛋白质芯片技术筛选壮族单纯性室间隔缺损(VSD)血清标志蛋白。方法:用表面增强激光解吸、电离飞行时间质谱技术和CM10芯片检测25例壮族单纯性VSD患儿与36例儿科常见病患儿血清,用Ciphergen公司的BiomarkWizard软件分析数据。结果:有12个蛋白具有统计学差异,其中质荷比(m/z)6640.784、8570.154、8698.278、2244.582、5321.868、6441.972和7973.3107个蛋白在VSD患儿血清高表达,质荷比(m/z)2213.579、4604.572、2142.465、9198.582和2169.2645个蛋白下调。结论:蛋白质芯片技术是发现与筛选室间隔缺损血清标志蛋白的快速、有效的蛋白质组学工具。
Objective： Using ProteinChip technology to screen serum protein markers in patients with Simple Ventricular Septal Defect （VSD）. Methods： Serum samples from 25 Zhuang simple VSD patients and 36 patients with common pediatric diseases were detected by surface-enhanced laser desorption/ionization timeof-flight mass spectrometry （SELDI-TOF-MS） technology and CM10 ProteinChip. Data were analyzed by Biomark Wizard software （Ciphergen Inc. ）. Result： Serum proteins were significantly different between VSD and control group. Mass to charge ratio（m/z）6 640. 784,8 570. 154,8 698. 278,2 244. 582,5 321. 868, 6 441. 972 and 7 973. 310 were of high expression in serum of VSD, and m/z 2 213. 579, 4 604. 572, 2 142. 465,9 198. 582 and 2 169. 264 were of lower expression in serum of VSD. Conclusion： ProteinChip technology is a quick and effective tool for proteomics, and it is capable of discovering and screening serum protein markers of VSD.
出处
《广西医科大学学报》
CAS
北大核心
2007年第6期832-835,共4页
Journal of Guangxi Medical University
基金
广西教育基金资助项目(桂教科研200420)
关键词
室间隔缺损
标志蛋白
蛋白质芯片技术
蛋白质组学
ventricular septal defect
protein biomarkers
ProteinChip technology
proteomics
