新生大鼠胰β细胞的发育及葡萄糖精氨酸对它的影响

A MORPHOLOGICAL STUDY ON β CELL IN PANCREAS ISLAND BY INJECTION OF GLUCOSE AND ARGININE IN NEONATAL RAT

本实验选用Wistar新生大鼠，出生后第2天开始腹腔注射葡萄糖或葡萄糖加精氨酸共6天，每日2次，对照组不做处理．分别于生后第10、20天剖腹取胰腺，制作光电镜标本，并用免疫组织化学PAP标记β细胞．同时测定β细胞及其分泌颗粒的数面密度（NA）和体密度（Vv）．结果显示：对照组第20天胰β细胞及其分泌颗粒的NA、Vv均明显高于第10天组；尽管实验组β细胞的NA、Vv低于对照组．但其β颗粒的NA、Vv却显著高于对照组。上述结果提示．大鼠生后发育期间胰岛β细胞及其分泌颗粒的数量、体积逐渐增加，而葡萄糖、精氨酸主要引起β细胞提前成熟。

Newborn Wistar rats were treated twice a day for 6 days after birth by glucose (G) or glucose with arginine (G + A ).The animals were killed on the tenth and twentieth day of life.The pancreas was observed by light and electronic mi-croscopy. The island β celIs were showed by the immunochis-tochemistry (PAP method) and its NA and Vv were measured.The NA and Vv of 6 granules were measured too. The result showed that the NA. Vv of-β cell and β granules were higer in normal rat on the twentieth day than the tenth day. A re markable rise in NA Vv of 6 granules were observed in G and G+A treated rat. It seemed that G and G+A could accelerate β granules maturation.