摘要
基于氯化血红素(Hemin)对鲁米诺-过氧化氢化学发光反应的高催化活性,建立了测定Hemin的高灵敏的流动注射化学发光新方法。Hemin的检出限为2.5×10-10g/ml,线性范围为2.0×10-9~4.0×10-7g/ml,相关系数为0.9991。本法干扰小,灵敏度高,为进一步建立以Hemin为标记物的发光免疫分析打下了基础。
Based on the high activity of hemin to the chemiluminescence reaction of luminol and hydrogen peroxide, the sensitive method of determination of peroxidase-like hemin is developed by flow injection chemiluminescence analysis. The detection limit for hemin is 2.5×10 10 g/ml. The linear range is 2.0×10 -9 ~4.0×10 -7 g/ml. The method can be applied to chemiluminescence immunoassay considered hemin as label.
出处
《南通医学院学报》
1997年第3期321-322,共2页
ACTA Academiae Medicinae Nantong