低强度激光对电刺激引起的C2C12自由基损伤的光生物调节作用

Effects of photobiological modulation of low intensity laser on the damage of free radicals of C2C12 caused by electrical stimulation

利用电刺激C2C12作为细胞模型，研究低强度激光对电刺激引起的C2C12线粒体功能自由基损伤的光生物调节作用。以45V 20ms 5Hz电刺激C2C12细胞，采用固定照射时间、不同照射强度的LIL，以荧光探针标记法检测电刺激及LIL对ROS含量的影响，研究LIL对电刺激后肌管ROS的代谢变化的影响。结果：1）ES 75min组与对照组比较ROS生成显著增加（P〈0．01），线粒体功能显著性下降（P〈0．05），电刺激引起的C1C12线粒体功能自由基损伤的细胞模型建立；2）0．33～8．22J／cm^2激光对正常培养细胞线粒体功能无影响，但可以改善电刺激引起的线粒体功能低下的状态，此剂量段为PBM的作用剂量范围；3）0.33～2．646J／cm^2激光可以促进细胞内稳态的康复，其机制主要与通过光化学作用提高机体的抗氧化作用有关。结果说明：适当剂量的低强度激光对骨骼肌细胞有光生物调节作用，是一种无损伤的促进运动性疲劳或自由基损伤康复的新型手段。

By utilizing electrically stimulated C2C12 as the cell model, the authors studied the effects of photobiological modulation （PBM） of low intensity laser （LIL） on the damage of functional free radicals of mitochondria of C2C12 caused by electrical stimulation. The authors applied electrical stimulation with such parameters as 45 V, 20 ms and 5 Hz to stimulate C2C12, tested the effects of electrical stimulation and LIL on the content of reactive oxygen species （ROS） by using fixed radiation time, LIL at different levels of radiation intensity and fluorescent probe marking method, studied the effects of LIL on the metabolic change of ROS of myotubes after electrical stimulation, and revealed the following findings：1） comparing the 75 min electrically stimulated group with the control group, the production of ROS is significantly increased （P〈0.01）, wile the functions of mitochondria are significantly weakened （P〈0.01）, which indicate that the cell model of the damage of functional free radicals of mitochondria of C2C12 caused by electrical stimulation is established; 2） laser at the energy level of 0.33-8.22 J/cm^2 has no effect on the functions of mitochondria of normally cultured cells, but can improved the condition of low performance of mitochondrial functions, this rang of energy level is the functional range of PBM; 3） laser at the energy level of 0.33-8.22 J/cm^2 can boost steady recovery within the cells, whose mechanism is chiefly related to enhancing the anti-oxidation function of organisms via photochemical action. The said findings indicate that an appropriate level of low intensity laser has the effects of PBM on skeletal muscular cells, being a new trauma free means that can boost the recovery of kinetic fatigue or free radical damage.