摘要
用lipofectin介导质粒pLXSN和人神经营养素3(hNT-3)的重组质粒pLhNT-3SN转染PA317细胞,获得了较高滴度的重组逆转录病毒,感染成肌细胞后用PCR和Northern杂交方法检测出neo基因与hNT-3的mRNA。
High titer recombinant retrovirus was obtained by transfecting PA317 cells with recombinant plasmid pLhNT 3SN using lipofectin. neo r Gene and the expression of hNT 3 mRNA were detectable in the infected myoblasts by using PCR and northern blot. The obtainment of engineered cells with high expression level depends on the selection of media and incubating temperature.
出处
《军事医学科学院院刊》
CSCD
北大核心
1997年第3期173-176,共4页
Bulletin of the Academy of Military Medical Sciences