髓过氧化物酶-463G/A基因多态性与冠状动脉疾病相关性研究

Association between myeloperoxidase-463G/A polymorphism and coronary artery disease

目的:通过对髓过氧化物酶-463G/A基因多态性分型,探讨其与冠状动脉疾病(CAD)易患性的关系。方法:采用病例-对照研究的方法。病例组79例,均行冠状动脉造影证实患有CAD;正常对照组69例,部分行冠状动脉造影证实冠状动脉正常,部分为门诊健康体检者,正常对照组所有入选病例均排除CAD史,脑卒中短暂性脑缺血发作病史及相似病史,排除周围血管疾病史。均用PCR-RFLP的方法判断各组基因型,最后进行统计学分析。结果:携带等位基因A的CAD发病率是携带基因G的0.433倍;病例组中造影结果不同的2组比较,携带至少1个A基因的冠心病的发生是不携带该基因的0.197倍。结论:髓过氧化物酶基因-463位点G/A多态性与CAD的易患性显著相关。该位点A基因的表达在CAD的发生过程中起保护性作用。

Objective：To study the relationship between myeloperoxidase -463G/A polymorphisms and susceptibility to CAD as well as the incidence of future cardiovascular events through determining the genotype of person who were recruited in case group and control group. Method：We performed case-control study. The case group consisted of 79 patients who had all angiographically proved CAD. The control group consisted of 69 individuals who did not have a history of CAD,stroke,transient ischemic attack, similar to cases, or peripheral vascular disease. Some of them had angiographically proved no CAD, the others were selected from healthy outpatients. We used PCR-RFLP method to decide the genotype of patients. At last we carried out statistical analysis. Result：We found the risk of CAD for carrying A allele was 0. 433 times of G allele. We divided case group into two group based on the results of coronary angiography. And we found the risk of coronary heart disease for CAD patients carrying at least one A allele genotype which was 0. 197 times of GG genotype. Conclusion： After analyzing we came to the conclusion that -463G/A polymorphism of the MPO gene influence the risk of CAD. And A allele had protective function in CAD occurrence.