喉癌组织中CD105标记的微血管密度VEGF及p53蛋白的相关性研究

Relevance of Endoglin(CD105) VEGF and p53 with invasion metastasis and prognosis of laryngeal carcinoma

目的:评价微血管密度(MVD)的临床意义,比较分析CD105、VEGF、p53蛋白之间的相关性,探讨三者在喉癌肿瘤血管生成中的相互关系及临床病理意义。方法:采用免疫组织化学技术检测以CD105标记的MVD(CD105-MVD)、VEGF、p53蛋白在喉癌组织中的表达。结果:40例喉癌组织CD105-MVD为14.90±7.40,明显高于对照组织(P<0.05);Ⅲ、Ⅳ期喉癌组织的MVD高于Ⅰ、Ⅱ期,颈淋巴结转移组高于无淋巴结转移组,其差异有统计学意义;VEGF阳性率77.5%。p53蛋白阳性率67.5%。VEGF表达与肿瘤的TNM分期及病理分化有显著相关性(P<0.05)。p53蛋白表达与淋巴结转移呈显著相关(P<0.05)。CD105-MVD与VEGF、p53蛋白之间呈明显正相关(r=0.641,P<0.01;r=0.534,P<0.01)。结论:CD105与肿瘤活性血管生成有密切联系,是肿瘤新生血管的标志;喉癌组织CD105标记MVD的测定可作为预测喉癌患者复发转移及评估预后的重要独立指标;喉癌中VEGF的表达、p53蛋白表达与CD105-MVD呈正相关,三者联合可作为判断喉癌侵袭转移及预后的重要指标。

Objective：To evaluate the clinical significance of microvessel density（MVD）and investigate the relationship of Endoglin（CD105）,VEGF and p53 protein and their significance of clinical pathology. Method： Pathologic paraffin-embedded tissuesand clinic data of 40 patients with primary squmamous cell carcinoma of larynx were studied. Serial sections were respectively stained with Endoglin（CD105）, VEGF and p53 by immunohistochemistry and its expressions were investigated. Microvessel density （MVD） highlighted by Endoglin（CD105）were counted according to a standard protocol. Result：Endoglin（CD105）expression in tumour tissue was significantly higher than in normal mucosa（P〈0.05）. The mean MVD value for Endoglin（CD105） was 14.90±7.40. The mean CD105-MVD value of T3 and T, tumours showed a significantly higher staining than that of T1 and T2 tumours; The mean CD105-MVD value of tumours with metastasis was also higher than that of tumours with no metastasis （P〈0.05） ; The expression of VEGF was observed in cytoplasm of tumour cell and its positive rate was 77.5% in laryngeal carcinoma, which was significantly correlated with TNM stage and pathological differentiation of laryngeal carcinoma（P〈0.05）. The expression of p53 protein was mainly observed in nucleolus of tumour cell and its positive rate was 67.5 %, which was significantly correlated with metastasis of lymph node. Positive relevance was found between CD105-MVD and VEGF（r=0. 641 ,P〈0.01）; Positive relevance was also found between CD105- MVD and p53 （ r= 0. 534, P〈0.01 ）. Conclusion： Endoglin（CD105） is a marker of tumour angiogenesis for itssignificant associated with active angiogenesis in laryngeal carcinoma. The study shows that CD105-MVD in laryngeal carcinoma is an independent indicator of predicting invasion,metastasis and recurrence , and evaluating prognosis of malignant tumours. CD105-MVD, VEGF and p53 could be important indicators to evaluate invasion,metastasis and recurrence of laryngeal carcinoma.