次声对大鼠十二指肠黏膜生长抑素及胆囊收缩素水平的影响

The effect of infrasound on the level of somatostatin and cholecystokinin in rat duodenal mucosa

目的研究不同强度和频率的次声作用对大鼠十二指肠黏膜生长抑素(SS)及胆囊收缩素(CCK)水平的影响。方法将140只雄性SD大鼠随机分为对照组、8Hz-90dB组、8Hz-130dB组及16Hz- 130dB组,每组35只。各次声作用组大鼠按设计要求分别暴露于相应频率和强度的次声环境中,每天2h;对照组大鼠于同一时间内亦置入次声舱中,每天2h,但未给予次声作用。各组分别于次声作用后第1,7,14,21及28天时各随机取出7只大鼠进行标本制备,应用放射免疫法测定十二指肠黏膜中SS、CCK的含量。结果各次声作用组与对照组比较,次声作用第1天时各组SS、CCK水平均无明显变化(P>0.05),作用第7,14,21及28天时均明显升高(P<0.05),其中以第14天时尤为显著,与第7,21及28天时比较,差异具有统计学意义(P<0.05);在第21及28天时,各组SS、CCK水平均有所下降,但仍显著高于对照组。除次声作用第1天外,8Hz-130dB组十二指肠黏膜SS、CCK含量在第7,14,21及28天时均明显高于8Hz-90dB组,16Hz- 130dB组十二指肠黏膜SS、CCK含量在第7,14,21及28天时均明显高于8Hz-130dB组。结论8Hz-90dB,8Hz-130dB或16Hz-130dB次声作用均可引起大鼠十二指肠黏膜SS及CCK水平升高,其影响程度与次声作用强度、频率及作用时间等密切相关,大鼠经次声多次作用后可产生一定适应性。

Objective To study the effects of infrasound of different frequencies and intensities on the levels of somatostatin （SS） and cholecystokinin （CCK） in rat duodenal mucosa. Methods A total of 140 male Sprague- Dawley rats were randomly divided into a control group, an 8 Hz-90 dB group, a 8 Hz-130 dB group and a 16 Hz - 130 dB group. The experimental groups were exposed to infrasound of 8 Hz-90 dB, 8 Hz-130 dB or 16 Hz-130 dB, respectively, for 2 hours a day, and the control group was put under sham infrasonic exposure. After being exposed for 1, 7, 14, 21 and 28 days, 7 rats were randomly selected from each group and underwent SS and CCK levels detection in duodenal mucosa by microarray radioimmunity method. Results Compared with the control group, the SS and CCK levels of duodenal mucosa did not change significantly after exposure for 1 day. The SS and CCK levels of duodenal mucosa significantly increased in 8 Hz-90 dB group, 8 Hz-130 dB group and 16 Hz-130 dB group after exposure for 7,14, 21 and 28 days（ P 〈0. 05 ） with a peak at the 14th day of exposure. At the 21 th and 28th days, the SS and CCK content were decreased in all experimental groups, the SS and CCK levels of duodenal mucosa in 8 Hz-130 dB group were significantly higher than those in 8 Hz-90 dB group, and the SS and CCK levels of duodenal mucosa in the 16 Hz-130 dB group were significantly higher than those in 8 Hz-130 dB group after being exposed for 1, 7, 14, 21 and 28 days. Conclusion lnfrasound can increase SS and CCK levels in duodenal mucosa of rats exposed to infrasound with 8 Hz- 90 dB, 8 Hz-130 dB or 16 Hz-130 dB. The SS and CCK levels in duodenal mucosa of rats were related to the frequency, intensity and action time of infrasound. The rats had adaptability to multi-exposure of infasound.