利用噬菌体快速鉴定沙门氏菌的研究

Study on the Rapid Identification of Salmonella by Using Bacteriophage

用肠杆菌科7个组合噬菌体对培养不同时间的60株肠杆菌科细菌进行平板法和液体法裂解,观察其裂解效果,并与生化鉴定进行比较。结果表明:平板法裂解培养2h、6h、10h和≥18h的沙门氏菌(生化鉴定为45株)裂解率分别为91.1%、93.3%、97.8%和88.9%,其余菌株几乎不被沙门氏菌特异噬菌体裂解;液体法裂解培养10h的菌液效果较佳。对150份食品样品检测也获得相似结果。说明肠杆菌科7个组合噬菌体对培养10h的沙门氏菌有较高的敏感性和特异性,这对实现沙门氏菌实时、快速而准确的检测有重要意义。

To study the incidence rate of Salmonella, seven strains bacteria-specific phages as a group were used for identification of Salmonella species. All of the 60 bacteria （for test） were inoculated in Luria Bertani （LB） broth, cultivated in 37℃ for 2 hours, 6 hours, 10 hours and≥ 18 hours respectively, then tested on agar plate and in LB broth, and made mlcro-biochemical identification through automatic biochemistry analyzer, to confer the most suitable inoculated hours for test bacteria. The result showed that, tested on agar plate, the positive rates of Salmonella cultivated for 2h, 6h, 10h and ≥ 18h were 91.1%, 93.3 %, 97.8 % and 88.9% respectively; while tested in LB broth, a reasonable result was showed in the 10h-inoculated tested bacteria. It was resemble to test with food samples bought from some markets in Nanjing. It provided theoretical evidence for performing detection Salmonella species in food with real time, speed, selective and specific.