植酸酶的纯化及其酶学性质初步研究

Studies on Purification and Properties of Phytase from Trichoderma viride

从绿色木霉LH374固态发酵产物中提取植酸酶。粗酶液经硫酸铵沉淀、凝胶过滤、离子交换层析纯化后,提纯倍数可达13.3,回收率为27.1%。酶学性质研究表明,植酸酶最适作用温度为55℃、最适作用pH为6.0,米氏常数Km为0.15mmol/L。

The phytase was extracted from solid state leavening of Trichoderma Hiride LH374. The crude product was purified by （NH4）2SO4 precipitation, gel filtration and ion exchange chromatography. The purified phyatse was 13.3 times of the raw products, and the extraction ration was 27.1%. The study on the enzymology of phyatse showed that the optimal temperature and pH were 55℃ and 6.0, respectively. The Km value of the phytase was 0.15mmol/L.