几株乳酸菌益生潜力及降胆固醇的研究

Study on the Probiotic Properties of Some Lactic Acid Bacteria Strains

采用体外试验方法研究7株乳酸菌和双歧杆菌对人体消化道不良环境的抵抗能力、在肠道上皮的黏附定植能力及其降胆固醇潜力。结果表明:受试菌对CCL-187细胞粘附能力存在显著差异(P<0.05),粪肠球菌M1粘附能力最强;各菌对胃液和胰液都有较强耐受能力,在模拟胃液和胰液中分别培养3h和4h后仍能保证95%以上存活;在含胆汁培养基中受到不同程度抑制,但都能存活;受试菌在生长过程中能够使环境中胆固醇降低从25%～54%不等,粪肠球菌A30和A31和嗜酸乳杆菌A878和PB1能使胆固醇降低40%以上,是良好的降胆固醇备选菌株。

The acid-and bile-tolerant, the transit tolerance, the ability to adhere to human intestinal cells and the ability to assimilate cholesterol of seven lactic acid bacteria and Bifidobacteria strains were studied by an in vitro method. The abilities to adhere to CCL-187 cells were different between all strains tested（ P 〈 0.05 ） and high attachment was observed with Enterococcus faecium M1. All strains tested were considered intrinsically resistant because the viability of them can remain 〉 95% when incubated in simulated gastric and small intestinal juice for 3h and 4h respectively. All strains have the ability to grow in the presence of bile, and the ability varied among the strains. The cholesterol assimilated ranged from 2.5% to 54%. Enterococcusfaecium A30, A31 and Lactobacillus acidophilus A878, PB1, which assimilated more than 40% cholesterol from cireurastances, can be good hypocholesterolemic candidate strains for functional food.