摘要
利用ERIC-PCR技术对苏云金芽孢杆菌(Bt)、蜡状芽孢杆菌(Bc)和对照菌基因组DNA进行扩增,回收、标记BtPCR扩增片段,分别与各菌株的基因组DNA进行斑点杂交和Southern杂交,筛选Bt标识序列。结果显示:Bt各菌株均可扩增得到250bp的特异片段;Bt和Bc均可得到600bp的共有扩增片段;以筛选得到的569bp片段为探针,可特异性地与Bt基因组DNA杂交;ERIC-PCR技术可以在DNA指纹图谱水平区分鉴别Bt与Bc菌,正确反映出两者的亲缘关系。结果表明ERIC-PCR技术在Bt的检测及在Bt与Bc的鉴定中具有较强的实用性。
To screen the identifier of B. thuringiensis, genomic DNA of B. thutingiensis, B. cereus and control strains were amplificated by ERIC- PCR. Fragments amplified from B. thuringiensis were retrieved, cloned, marked by α-^32 P, dot blot and southern hybridized with genomic DNA of these strains. It shows that every strains of B, thuringiensis can be amplified to produce a 250bp fragment, and beth B. thuringiensis and B. cereus can be amplified to produce a 600bp fragment. Hybridization result between 569bp probe and genomic DNA of B. thuringiensis shows a high specificity. Discrimination and genetic relationship between B. thuringiensis and B. cereus can be revealed by ERIC fingerprint. This research indicates that ERIC-PCR technique is a practical method in the detection and characterization of B. thuringiensis and B. cereus.
出处
《微生物学通报》
CAS
CSCD
北大核心
2007年第6期1184-1187,共4页
Microbiology China
基金
辽宁出入境检验检疫局资助项目(No.LK-30-2002)
