摘要
采用斑点酶联免疫分析（ｄｏｔｉｍｍｕｎｏｂｉｎｄｉｎｇａｓｓａｙＤＩＢＡ）技术，对海王“金牡蛎胶囊”中牡蛎精粉进行鉴别，在每点６ｎｇ蛋白以下，不与其他７种贝类交叉。用酶联免疫吸附试验（ＥＬＩＳＡ），牡蛎蛋白对照品０．０５μｇ～０．９０μｇ呈线性，ＲＳＤ为６．６％，平均回收率１１１％。表明酶免疫技术可以在１０－８ｇ以上的分析水平。
WTBZ〗 By using dot immunobinding assay, the Concha Ostreae powder in Haiwang Jinmuli Capsule and other 7 preparations of same kind were identified below 6ng protein, and enzyme linked immunosorbent assay was used to determine the specific protein in the preparations.A good linearity was shown at the concentration of 50 to 900ng. Average recovery is 111% and RSD is 6.6% (n=4). It is suggested that the methods for the quality control have been improved from the level of 10 -4 to 10 -8 g. [WTHZ〗
出处
《中药新药与临床药理》
CAS
CSCD
1997年第3期159-161,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省自然科学基金
关键词
牡蛎制剂
酶免疫技术
质量
中药制剂
WTBZ〗@ Concha Ostreae Capsule/chemistry
@ Specific Protein/analysis
@ dot immunobinding
Enzyme-Linked immunosorbent assay
