天然脑活素促细胞增殖和抗细胞衰老作用的研究

Study on the Effects of Natural Cerebrolysin on Cell Proliferation Activity and Replicative Senescence and its Mechanism

目的:探讨天然脑活素对人胚肺二倍体成纤维细胞(MRC-5)增殖能力和复制性衰老的影响及其机理。方法:光镜下观察MRC-5细胞衰老表型,常规计数细胞代龄、传代速度,MTT法检测细胞增殖活度,X-Gal染色法观察细胞衰老情况,计数衰老细胞百分率。结果:天然脑活素可维持细胞的非衰老表型,增加MRC-5细胞代龄,天然脑活素孵育MRC-5细胞的细胞增殖能力较空白老龄对照组明显升高(P<0.05),细胞的增殖速度显著加快。另外,天然脑活素连续培养的细胞在老龄阶段衰老细胞数显著低于老龄对照细胞(P<0.01)。结论:天然脑活素可升高MRC-5细胞增殖能力,有效延缓细胞的复制性衰老。

Objective To explore the effects of Natural Cerebrolysin on cell proliferation activity and replicative senescence of human embryonic lung diploid fibroblasts （MRC-5 cells） and its mechanism. Methods To observe the changes of senescent phenotype by light microscope, count the passage ages and velocity, detect the potential of cell proliferation by MTT. The changes of cells senescence of Natural Cerebrolysin-effected MRC-5 cells were studied by X-Gal staining method, and the positive cell rates of senescence cells were detected and analyzed.. Results Natural Cerebrolysin maintained unaltered cell morphology and increased life span of MRC-5 by at least 18-21 passages. Comparing with the control senium cells, the proliferation potential of MRC-5 cells incubated with Natural Cerebrolysin was increased obviously （P 〈 0.05）, and the passage velocity was enhanced double of that of the control senium cells （P 〈 0.01）. In addition, the positive cell rate of senescence cells was significantly lower in late passage cells in sequenced multigeneration culture in Natural Cerebrolysin than those of control senium cells （P 〈 0.01）, and similar to those of young cells. Conclusion As a result, we concluded that Natural Cerebrolysin could notably promote the proliferation activity of MRC-5 cells and delay the cells replicative senescence efficiently.