Cdk3在结肠癌变过程中的表达及其意义

Expression of Cdk3 in colon carcinogenesis and its significance

目的:研究Cdk3在结肠息肉、结肠腺瘤、结肠癌组织中的表达,探讨Cdk3在结肠癌变过程中的演变规律及其意义.方法:用免疫组织化学和原位杂交的方法检测22例结肠癌组织、22例结肠腺瘤组织、24例结肠息肉组织中Cdk3蛋白和mRNA的表达.结果:Cdk3蛋白和mRNA在结肠息肉、结肠腺瘤、结肠癌组织中的表达呈核.质型,以质为主.图像分析结果显示,结肠癌组织中Cdk3蛋白表达平均光密度值(0.80±0.36)明显高于结肠腺瘤组织(0.48±0.22)和结肠息肉组织(0.25±0.13)(P<0.05),结肠腺瘤组织中Cdk3蛋白表达平均光密度值明显高于结肠息肉组织(P<0.05),结肠癌组织中的阳性细胞数(266.5±40.2)明显高于结肠腺瘤组织(132.0±37.4)和结肠息肉组织(129.3±26.7).结论:Cdk3在结肠癌变过程中表达逐步增强,在结肠癌组织中异常高表达,可能在结肠癌变过程中发挥重要作用.

AIM： To investigate the expression profile of Cdk3 in colon poplyp, adenoma and its adjacent tissues and its significance in the evolution of colon cancer. METHODS： Using immunohistochemistry and in situ hybridization, Cdk3 protein and mRNA expressions were detected in 22 cases of colon cancer, 22 cases of colonic adenoma and 24 cases of colonic polyp. RESULTS： Immunohistochemistry and in situ hybridization showed Cdk3 protein in the cyto-plasts and cytoplasms of colonic polyp, colonic adenoma and cancer tissues. Cdk3 was mainly expressed in cytoplasm. Image analysis revealed that the mean optical density of Cdk3 protein in colon cancer tissue （0.80±0.36） was significantly higher than that in colon adenoma （0.48±0.22） and polyp tissues （0.25±0.13, P 〈 0.05）, and the mean optical density of colonic adenomas was significantly higher than that of colon polyps （P 〈 0.05）. The results of in situ hybridization showed significantly more ceils expressing Cdk3 rnRNA in colon cancer tissue （266.5±40.2） than in colon adenoma （132.0±37.4） and polyp tissues （129.3±26.7, P 〈 0.05）. CONCLUSION： Cdk3 expression gradually increases during the course of colon cancerization. Cdk3 may be involved in colon carcinogenesis.