鲜壁虎不同部位提取液对小鼠H22肝癌体内外抑制作用

Anti-tumor effects of fresh extracts from different parts of gecho on mouse H22 hepatocellular carcinoma in vivo and in vitro

目的:探讨鲜壁虎不同部位提取液对培养的H22肝癌细胞体外抑制增殖作用和对H22实体型移植瘤小鼠的体内抑制作用,寻找有效的抗肿瘤活性成分.方法:MTT法检测高、低剂量的壁虎皮肤、头部、肌肉骨骼、内脏、尾部及全壁虎的提取液对H22肝癌细胞的体外杀伤作用.采用昆明小鼠H22移植瘤模型观察壁虎内脏高、低剂量组,去内脏壁虎高、低剂量组,全壁虎高、低剂量组的体内抑瘤活性及荷瘤小鼠胸腺和脾脏指数的变化.结果:体外实验结果显示,壁虎内脏提取液高剂量可显著抑制H22细胞的增殖,抑制率为61.6%,与其他部位的提取液相比有显著差异(P<0.01).体内抑瘤实验,5-FU组的抑瘤率为65.3%,内脏低剂量和高剂量组的抑瘤率为8.4%和16.8%,去内脏为26.3%和42.1%.全壁虎为31.6%和52.1%.去内脏壁虎的高、低剂量组和全壁虎的高、低剂量组的瘤重与模型组比较有统计学差异(P<0.05或P<0.01).全壁虎高剂量组与5-FU组的瘤重相比较无统计学差异.各壁虎组与模型组小鼠的胸腺重、胸腺指数,脾脏重、脾脏指数相比均没有明显差异.而5-FU组胸腺重、胸腺指数,脾脏重、脾脏指数较模型组和各壁虎组均明显降低(P<0.01).

AIM： To research the anti-proliferation effects of fresh extracts from different parts of gecko on cultured H22 hepatoma cells in vitro, and the tumor-inhibiting effects of these extracts on mice with transplanted H22 tumors in vivo, to identify the active components. METHODS： MTT assay was used to evaluate the anti-proliferation effects of fresh extracts from different parts of gecko, such as the skin, head, muscle and bone, internal organs, tail and whole gecko, on cultured H22 hepatoma cells in vitro. Transplanted H22 tumor-bearing mice were used as a model for observing the anti- tumor effects of extracts from gecko internal organs, geckos without internal organs and whole geckos. The mice were divided into two groups and given each type of extract, with one group receiving a high dose and the other a low dose. RESULTS： High doses of gecko intemal organ extract revealed an obvious anti-proliferation effect on H22 cells in the in vitro experiment, the inhibition rate was 61.6%. Compared with other extracts, the inhibitory effect was significant （P 〈 0.01）. In the in vivo experiment, the level of inhibition in the 5-FU group was 65.3%. The levels of inhibition of low and high doses of extracts from gecko internal organs extract were 8.4% and 16.8%, respectively. Those seen with low and high doses of extracts from geckos without internal organ were 26.3% and 42.1%, respectively, while those low and high doses of extract from whole gecko were 31.6% and 52.1%, respectively. Compared with the model group, there were statistical differences in tumor weights of mice treated with extract from geckos without internal organs and whole gecko （P 〈 0.05 or P 〈 0.01）. However, compared with the tumor weights of the 5-FU group, only the high-dose of extract from whole gecko showed no difference （P 〉 0.05）. There were no differences in thymus weight, thymus index, spleen weight, or spleen index all gecko groups and the model group. However, these four observed items were all significantly lower in the 5-FU group than those in the model group and whole gecko group （P 〈 0.01）. CONCLUSION： Gecko extracts have good antitumor effects.