血管紧张素原基因T174M变异与肝硬化的相关性

Association analysis of T174M variants of the angiotensinogen gene with hepatocirrhosis

目的：研究血管紧张素原（angiotensinogen，AGT）基因T174M分子变异与肝硬化的关系．方法：提取正常人64例和肝硬化患者65例白细胞基因组RDNA．通过PCR、限制性片段长度多态性和测序等技术，观察AGT基因型在肝硬化组和正常组分布的差异．结果：AGT基因T174M位点MT和TT基因型的频率在正常组和肝硬化组分别为82．8％、17．2％和84．6％、15．4％，两组之间不存在差异（Χ^2=0．077，P〉0．05）．结论：血管紧张素原基因T174M变异与肝硬化没有显著关系．

AIM： To detect the relationships between variants in the T174M polymorphism of the angiotensinogen gene and hepatocirrhosis. METHODS： Genomic DNA from leukocytes was analyzed for genetic variants at the T174M polymorphism of the AGT gene by polymerase chain reaction （PCR）, restriction fragment length polymorphism （RFLP） and automatic sequencing. RESULTS： The distributions of MT and TT genotypes were not different between the hepatocirrhosis group （84.6%, 15.4% ） and the control group （82.8%, 17.2%） （Χ^2 = 0.077, P 〉 0.05）. CONCLUSION： T174M polymorphism in the AGT gene is not likely to be involved in hepatocirrhosis.