Vimentin在胰腺癌细胞中的表达及临床意义

Vimentin expression in pancreatic cancer and its clinical significance

目的:研究vimentin在胰腺癌细胞中的表达及意义.方法:采用免疫组织化学的方法检测99例胰腺癌组织中vimentin的表达,并分析其与临床病理资料的关系.免疫荧光、Westem blot分析不同胰腺癌细胞中vimentin的表达,Transwell侵袭小室法检测细胞的运动能力.结果:在99例胰腺癌组织中vimentin阳性16例(16%),vimentin的阳性表达和淋巴结转移(P= 0.02)、远处转移(P<0.001)、肿瘤细胞的分化程度(P=0.002)存在明显的相关性.Vimentin在低分化的胰腺癌细胞Panc-1中呈高表达,而在中分化和高分化的胰腺癌细胞AsPC-1和BxPc-3中表达较低.vimentin的表达和细胞体外运动能力相关.结论:胰腺祖细胞标记物vimentin在胰腺癌细胞中存在表达,胰腺癌的发生和干细胞可能存在一定的联系,并可能预测胰腺癌的恶性程度.

AIM： To investigate the expression of vimentin in pancreatic cancer and its significance.METHODS： Vimentin expression was examined in 99 cases of pancreatic carcinoma with immunohistochemistry, and the relationship between vimentin expression and clinicopathology were analyzed. Vimentin expression was investigated by immunofluorescence and Western blot in three pancreatic cancer cells lines： Panc-1, AsPC-1 and BxPc-3. Transwell migration assay was used to analyze the migratory ability of different cells.RESULTS： Vimentin was detected in 16 cases （16%） of pancreatic cancer. Significant correlation was detected between the expression of vimentin and lymph node metastasis （P = 0.02）, distant metastasis （P 〈 0.001） and poor dedifferentiation （P = 0.002）. Vimentin was highly expressed in poorly differentiated pancreatic cancer cells, Panc-1. Besides, vimentin expression was correlated with in vitro cell migration.CONCLUSION： Vimentin expression in pancreatic cancer cells has been identified. The histopathogenesis of pancreatic cancer may be correlated with stem cells. Vimentin may be a marker for predicting the malignancy of pancreatic cancer.