胞苷发酵培养基的优化研究

Culture Medium Optimization for Cytidine Production

采用响应面分析的方法对发酵生产胞苷的培养基进行优化,通过二水平正交试验考察葡萄糖、玉米蛋白粉、玉米浆、K2HPO4、尿素、起始pH值和发酵温度对发酵生产胞苷的影响,利用极差分析寻找发酵生产胞苷的主要影响因子,利用中心组合设计与响应面分析进一步考察主要影响因子并确定了最佳培养基的组成.结果表明,发酵生产胞苷的主要影响因子分别为K2HPO4、玉米浆和葡萄糖.在优化培养基中,胞苷产量增加2倍,达到1.898 g.L-1,试验值与预测值基本相符.

Response surface analysis（RSA） was applied to optimize the culture medium for cytidine production.In the first optimization step,the influence of glucose,corn gluten meal,corn steep liquor,K2HPO4,urea,initial pH and fermentation temperature on cytidine production was evaluated using a fractional factorial design.The results show that the significant factors at the analysis range are：K2HPO4,corn steep liquor and glucose,respectively.Using the yield of cytidine as the optimization index,under optimal conditions,the experimental values agreed with the predicted values.The yield of cytidine increased more than 2 times to 1.898 g·L^-1.