湖南省2006年肾综合征出血热病原学监测研究

Virological surveillance on hemorrhagic fever with renal syndrome in Hunan province in 2006

目的了解湖南省啮齿动物中汉坦病毒带病毒率、基因型别和分布。方法采用直接免疫荧光(DFA)法检测鼠肺汉坦病毒抗原,从汉坦病毒阳性鼠肺标本中提取病毒RNA,应用汉坦病毒型特异性引物进行逆转录聚合酶链反应(RT-PCR)扩增、核苷酸序列测定以及基因分型和同源性分析。结果总鼠密度为3.15%,鼠带病毒率为1.31%,在室内捕获的7只褐家鼠、黑线姬鼠、小家鼠、黄胸鼠鼠肺标本中均发现携带SEO型病毒,在室外捕获的1只黑线姬鼠鼠肺标本则携带HTN型病毒。成功对6份阳性PCR产物进行了序列分析,浏阳市1、2、4、6号标本同源性较高为100%,邵东市的7、8号标本同源性也较高为100%,但两地区间的同源性差异为89.3%,L99株与浏阳分离株的同源性为94.4%,与邵东分离株的同源性为88.3%。结论湖南省汉坦病毒存在HTN和SEO型,以SEO型为主。

Objective To understand the rate of viral carrying status among rodents as well as genotypes and distribution of Hantaviruses（HV） isolated in Hunan province. Methods With DFA, the HV antigen in lung tissues of rodents was detected. The total viral RNA was extracted from the lung tissues of the HV infected rats and amplified with reverse transerition-polymerase chain reaction（ RT-PCR）, using the HV genotype specific primers. The amplified genes were then sequenced and subjected to genotyping and homologic analysis. Results The average density of rodents was 3.15 % and the virus carrying rate among rodents was 1.31%. Data from genotype analysis showed that the HV isolated from seven lung specimens taken from Rattus norvgicus , Apodemus agraius , Mus musculus , Rattus flavipectus among indoor rodents in Shaodong and Liuyang belonged to HV type II （SEOV）, and one isolated from Apodemus agraius in Shaungfen belonged to HV type I （HTNV） among outdoor rodents. Six strains were sequenced successfully and the homology between six srains was 88.3 %- 100 %. The homology of HN1, HN2, HN4, HN6 came from Liuyang and the HN7 and HN8 from Shaodong were both 100 % while the homology between L99 and the strains from Liuyang and Shaodong were 94.4 % and 88.3 % respectively. Conclusion HV type Ⅱ （SEOV） and the HV type I （HTNV） were all existed in Hunan province while SEOV was the main genotype.