亚慢性钐染毒对雄性小鼠精子质量的影响

Effect of Subchronic Samarium Exposure on Sperm Quality of Mice

为探讨稀土元素钐对雄性小鼠精子的毒性作用,让5组小鼠自由饮用含硝酸钐0,5,50,500,2000 mg.L-1的溶液3个月后,观察钐对小鼠精子活力、数量、畸形率及顶体完整率的影响。结果表明:各处理组精子活力、数量都有所降低,其中2000 mg.L-1剂量组与对照组相比,各指标值均有极显著差异(P<0.01);50,500,2000 mg.L-1剂量组的精子畸形率与对照组相比也具有极显著差异(P<0.01),且染毒剂量与精子总畸形率呈现出直线变化的剂量对数-效应关系。因此精子畸形率可作为稀土元素钐作用于雄性生殖系统的标志指标;各处理组的顶体完整率也均低于对照组。说明亚慢性钐暴露对小鼠精子具有一定的毒性作用。

To study the toxicity of samarium nitrate on male mice sperm quality, samarium nitrate was administrated to mice at different doses （0, 5, 50, 500, 2000 mg·L^-1） for 3 months. The spermic motility, spermic count, spermic abnormality rate and spermie acrosomal integrity were examined. The results showed that the spermic motility and count decreased in each group of treatment. All index in 2000 mg·L^-1 dose group shared a significant difference from the control group. The total spermie abnormality rate in 50, 500,2000 mg·L^-1 dose groups were all higher than the control group. And there was a dose-response relationship between dose logarithm of samarium nitrate and total spermic abnormality rate. The rate of spermic acrosomal integrity in each group of treatment were lower than the control group. High dose subchronic samarium exposure had definite toxicity to gonad of mice and the spermic abnormality rate could be considered as an index of rare earth element samarium action on testis.