甲藻单个细胞DNA的制备及在赤潮藻类分子鉴定中的应用

Method for PCR Amplification of Dinoflagellate rDNA with Individual Cells and Its Applification in the Identification of “Red Tide” Algae Species

报导一种赤潮甲藻单个细胞ＤＮＡ的制备方法．该法仅需１或数个甲藻细胞，在常规实验条件下，２０～３０ｍｉｎ即可完成整个过程．此方法的建立，可直接对自然种群进行分子分析，避免了实验室培养种与自然种群的差异，同时，使材料的来源并不依赖于甲藻培养技术的完善．最后。

A method for PCR amplification of Dionoflagellate rDNA with individual cells was reported and its applification for the identification of “red tide” algae species was discussed. It was demonstrated that the single cell PCR method was useful in analysing natural populations.This method eliminated the differences between lab investigation and natural population, and solved the problems resulting from the analysis of rRNA gene for those organisms which is difficult or even impossible to propagate in the lab.