ACC氧化酶的测定方法

A Kind of Assay Methods for ACC Oxidase

利用番木瓜ＣａｒｉｃａＰａｐａｙａＬ．果实为材料，研究了１－氨基环丙烷－１－羧酸（ＡＣＣ）氧化酶的体内和体外测定方法．在ＡＣＣ氧化酶的体外分析中，甘油体积分数为１０％～３０％时能稳定酶的活性；φ（ＣＯ２）为０．３％～２０％（反应体系气相中）和０．５～１２ｍｍｏｌ／ＬＡＣＣ提高酶的活性；较适宜的ｐＨ值为７．２．

Using papaya( Carica papaya L.)fruit as the experimental material, assay methods for l aminocyclopropane l carboxylate(ACC)oxidase in vivo and in vitro were studied. In vitro, ACC oxidase activity could be stabilized by 10%～30% φ (glycerol), and could be increased by 0.3 %～20% φ (CO 2)(in the gas phase of reaction system) and by 0.5～12 mmol/L ACC. The optimum pH was 7.2 for ACC oxidase in vitro .