HPLC法测定复方甲硫氨酸葡萄糖注射液中维生素B_1、维生素B_3和核黄素磷酸钠的含量

Determination of Vitamin B_1、Vitamin B_3 and Riboflavin Sodium Phosphate in Trivitamins and Diaminoacids Injection by HPLC

目的:建立一种HPLC法测定复方甲硫氨酸葡萄糖注射液中维生素B1、维生素B3和核黄素磷酸钠含量的方法。方法:采用C18柱,以乙腈-0.003mol.L-1己烷磺酸钠溶液(用冰醋酸调节pH值至2.6)(8:92)为流动相,检测波长270nm,流速为1.0ml.min-1。结果:样品中三种成分完全分离且线性关系良好,维生素B1、维生素B3和核黄素磷酸钠的回收率分别为100.3%、99.7%和100.1%(n=9)。结论:本法简单,准确,可用于复方甲硫氨酸葡萄糖注射液的质量控制。

Objective： To establish a method for determining Vitamin B1,Vitamin B3 and Riboflavin Sodium Phosphate in Trivitamins and Diaminoacids Injection by HPLC. Methods：Using C18 column,acetonitrile-0. 003 mol·L^-1 of sodium 1-Hexanesulfonate solution（adjusted to pH 2.6 by glacial acetic acid）（8： 92）as mobile phase,flow rate was 1.0ml·min^-1 ,wavelength of detector was 270nm. Result：Three components were completely separated and determined. The average recoveries （n= 9）were 100. 3% for Vitamin B1, 99.7% for Vitamin B3 and 100.1% for Riboflavin Sodium Phosphate. Conclusions：This method is simple,accurate and can be used for controlling quality of Trivitamins and Diaminoacids Injection.