高浓度二氧化碳对药材甲和烟草甲乙酰胆碱酯酶活性的影响

Effect of carbon dioxide enriched atmosphere on the activity of acetylcholinesterase in adults of Stegobium paniceum and Lasioderma serricorne (Coleoptera:Anobiidae)

用高浓度CO2气体胁迫处理药材甲Stegobium paniceum和烟草甲Lasioderma serricorne成虫，通过终点比色法测定不同处理时间下两种昆虫乙酰胆碱酯酶AChE的活力变化及其动力学响应，分析高浓度CO2气调胁迫与AChE性质之间的关系。结果表明，气调处理18h，药材甲和烟草甲比活力分别由对照1．22μmol／mg pro./30min和1．856μmol／mg pro./30min上升到2．231μmol／mg pro./30min和2．135μmol／mg pro./30min。处理6h内，二者AChE比活力均迅速升高，药材甲AChE在处理9-18h活力上升趋于平缓，而烟草甲AChE活力则在处理6-15h内稍微降低。药材甲和烟草甲分别在处理6h和9h内，其AChE动力学参数Km下降到最低点1．830μmol／L和7．632μmol／L，随着处理时间延长，其Km值回升，至18h逐步恢复至对照组水平。高浓度CO2气调胁迫处理亦导致药材甲和烟草甲AChEVmax的改变。

On stress of CO2 enriched atmospheres, the change of activity of acetylcholinesterase （ACHE） in adults of Stegobium paniceum and Lasioderma serricorne and their kinetic indexes were investigated by endpoint method colorimetry. The specific activity of S. paniceum and L. serricorne increased from control 1. 221 μmol/mg pro./30 min and 1. 856 μmol/mg pro./30 min to exposed for 18 h 2. 231 μmol/mg pro. / 30 min and 2.135 μmol/mg pro./30 min respectively. Increasing specific activity of AChE was noted both insects exposed to CO2 enriched atmosphere within 6 h. There was still slowly growing of specific activity for S. paniceum exposed to the atmosphere for 6 to 18 h. However, a gently decreasing trend was noted for L. serricorne exposed to the atmosphere for 6 to 15 h. The gm of AChE got the smallest values （1. 830 μmol/L and 7.632 μmol/L） for S. paniceum and L. serricorne treated within 6 and 9 h respectively. But after that, they recovered to normal control pests＇ level. Moreover, the Vmax of AChE from the two pests was also affected by control atmosphere. Therefore, the conclusion can be drawn that both S. paniceum and L. serricorne can acclimatize themselves to adverse condition （ i. e. stress of CO2 enriched atmosphere） by adjusting of character of ACHE.