盐酸戊乙奎醚对围体外循环期大鼠肠粘膜屏障功能的影响

Effects of penehyclidine on the intestinal mucous membrane barrier function in rats undergoing cardiopulmonary bypass

目的探讨不同剂量盐酸戊乙奎醚对围体外循环（CPB）期大鼠肠粘膜屏障功能的影响。方法雄性SD大鼠30只,体重300～400g,随机分为5组（n=6）：假手术组（S组）、CPB组、高剂量盐酸戊乙奎醚组（PH组）、中剂量盐酸戊乙奎醚组（PM组）和低剂量盐酸戊乙奎醚组（PL组）。建立大鼠CPB模型,S组仅置管不转流,PH组、PM组、PL组和CPB组分别在预冲液中加入盐酸戊乙奎醚2、0.6、0.2mg/kg和等容量生理盐水。停CPB后2h取大鼠肠系膜上静脉及腔静脉血,测定血浆D-乳酸、内毒素（LPS）浓度和二胺氧化酶（DAO）活性,透射电镜下观察小肠粘膜病理学结果。结果与S组比较,CPB组、PH组、PM组和PL组血浆D-乳酸、LPS浓度和DAO活性升高（P〈0.05）;与CPB组比较,PH组和PM组血浆D-乳酸、LPS浓度和DAO活性降低（P〈0.05）;与PL组比较,PM组和PH组血浆DAO活性、D-乳酸和LPS浓度降低（P〈0.05）;与PM组比较,PH组血浆DAO活性和D-乳酸浓度降低（P〈0.05）。PH组和PM组小肠上皮病理损伤程度较CPB组和PL组明显减轻。结论预充液中加入盐酸戊乙奎醚0.6mg/kg或2mg/kg可减轻围CPB期大鼠肠粘膜屏障功能的损伤,且呈剂量依赖性。

Objective To investigate the effects of different doses of penehyclidine on the intestinal mucous membrane barrier function in rats undergoing cardiopulmonary bypass （CPB）.Methods Thirty male SD rats weighing 300-400 g were randomly divided into 5 groups （n=6 each）：groupⅠsham operation （S）;groupⅡCPB;groupⅢ-ⅣCPB＋penehyclidine 2.0,0.6 and 0.2 mg/kg respectively,groupⅠserved as control.In groupⅡ-Ⅴthe animals underwent 1 h CPB.Blood samples and a 3 cm segment of ileum 5 cm from ileo-cecum were obtained at 2 h after CPB for determination of plasma diamine oxidase （DAO） activity and D-lactate and LPS concentrations and microscopic examination.Results Plasma DAO activity and D-lactate and LPS concentrations were significantly higher in groupⅡ-Ⅴthan in control group （groupⅠ）.Plasma DAO activity and D-lactate and LPS concentrations were significantly lower in groupⅢandⅣthan in groupⅡandⅤand were lowest in groupⅢ（penehyclidine 2.0 mg/kg） as compared with groupⅡ,ⅣandⅤ.Electron microscopy revealed that intestinal ultra-micro structural damage was significantly attenuated in groupⅢandⅣ.Conclusion Penehyclidine 0.6 and 2.0 mg/kg can protect the intestinal mucous membrane barrier function during CPB in a dose-dependent manner.