表面等离子共振生物传感系统构建及应用研究

Construction and Application of a Surface Plasmon Resonance Biosensor System

本文构建了以TI—Spreeta传感器为基础敏感元件、集微流道系统和数据信号处理电控盒于一体的表面等离子共振生物传感分析系统。在乙醇体积分数0．00～0．60浓度范围内检测乙醇标准溶液时，体积分数改变0．1，将引起共振像素位置变化约9．4个像素数目，表明传感系统的可逆性和重复性良好。采用自组装成膜技术制备了传感器敏感膜，观察了乙肝表面抗原和乙肝表面单克隆抗体的结合、解离以及传感器敏感膜的再生等动态变化过程。与传统的酶联免疫检测法相比，本方法具有无需酶标记、灵敏准确、快速，能够实现在线连续监控检测等优点，在食品安全、环境监测、药物筛选和生物医学研究中具有较大的应用潜力。

A simple and portable surface plasmon resonance （SPR） biosensor system is constructed, which mainly contains a basic sensing element of TI-Spreeta sensor, a microfluidic cell and a digital signal processor （DSP） . By measuring the refractive index （RI） of ethanol standard solutions of different volume fraction, the properties of reversibility, sensitivity, repeatability describing the performance of the biosensor system are analyzed. By measuring the Rt of ethanol solutions, the results show that the biosensor system has good reversibility and sensitivity. When the ethanol solutions in the volume fraction Tange from 0.00 to 0.60, the resonant pixel will shift 9.4 pixels corresponding to the change of 0.1 volume fraction. In addition, a sensing film is firstly immobilized on the sensor Au surface by using self-assembled monolayer （SAM） technology, and then a series of dynamic change processes on the affinity interaction between mHBsAb and HBsAg including association, dissociation and regeneration of sensor sensing film are investigated. Comparing with the conventional ELISA method, SPR technology possesses potential applications in the fields of food safety, environmental monitoring, drug discovery and biomedical science due to its typical advantages such as label-free, high sensitivity and accuracy, rapid, real-time continuous monitoring, etc.