鹿茸多肽促进肝细胞增殖及肝部分切除小鼠的肝再生

Promotion Effect of Velvet Antler Polypeptides on Proliferation of Hepatic Cells and Liver Regeneration

目的 研究鹿茸多肽（VAP）对细胞增殖及实验性肝再生的影响。方法 培养人胚肝细胞株（L-02）和原代大鼠脾细胞，体外加入不同剂量VAP，用MTT比色法观察其对细胞增殖的影响。整体观察VAP促进肝部分切除小鼠肝再生作用。结果 VAP50和25mg·kg^-1·d^-1能明显促进小鼠肝切除后的肝再生，与对照组比较差异显著（P〈0．01和P〈0.05）。VAP5～80mg·L^-1对L-02细胞株和大鼠脾细胞均有明显的促增殖作用。结论 VAP通过促进肝细胞增殖和增强免疫力的影响来加速实验性肝切除小鼠的肝再生。

OBJECTIVE To study the effects of velvet antler polypeptides（VAP） on the proliferation of hepatic cells and liver regeneration. METHODS L-02 cell line and rat spleen cells were cultured in different concentration of VAP for 24 h. MTF colorimetry was used for assaying proliferation. Mice modles of liver partial hepatectomy was established to study the effects of VAP on liver regeneration . 70 Mice were randomly divided into 5 groups. Mice in the VAP groups were treated with VAP at a dose of 12. 5,25 and 50 mg . kg^-1 . d^-1 for 3 d subcutaneously. At the same time, mice in the control group were given equivalent amount of 0. 9% sodium chloride solution and mice in the positive control group were given 200 mg . kg^- 1 . d^- 1 BDD subcutaneously. After 12 h of the last administration,all mice were sacrificed. The relative liver weight was obtained. RESULTS VAP 10 and 20 mg . L^-1 VAP showed significantly proliferation-promoting activity for L-02 cell line and rat spleen cells and increased incorporation of MTT colorimetry from （0. 969 8 ± 0.0716;0.8108±0.075 1） Bq （ control group） to （1.2188±0.1533;1.0217±0.111 2） and （1.5677±0.0849;1.3498± 0. 067 9） Bq, respectively （ P 〈 0. 01 or P 〈 0. 001 ）. Compared with the control group, VAP 50 and 25 mg . kg^-1. d^-1 significantly stimulated liver regeneration in mice excised partial liver （P 〈 0. 01 ）. CONCLUSION VAP showed a remarkable enhance effect on the liver regeneration after hepatectomy by stimulating proliferation of hepatic cells.