HPLC-ELSD测定赖氨肌醇维生素B_(12)口服液中肌醇含量

Determination of Inositol in Lysine,Inositol and Vitamin B12 Oral Solution by High Performance Liquid Chromatography with Evaporative Light-Scattering Detector

目的 建立简便、快速的HPLC—ELSD测定复方制剂中肌醇含量。方法 采用Kromasil NH2色谱柱，流动相为甲醇-水（60：40），流速0．8mL·min^-1，柱温室温。结果 肌醇在0．157～2．51g·L^-1。内线性关系良好（r=0．9996），最低检测限为39．3mg·L^-1，平均回收率99％，RSD为1．1％。在该奈件下，样品中辅料及其他成分不干扰肌醇测定。结论 本方法简便、快速、灵敏，为肌醇的质量控制提供了一种新的可靠分析手段。

OBJECTIVE To establish a simple and rapid HPLC-ELSD method for the analysis of insoitol of compound preparation. METHODS The separations were performed on a Kromasil NH2 column, with the mobil phase of methanol-water （60： 40）, at a flow rate of 0. 8 mL . min^ - 1. RESULTS The calibration curve was linear in the range of 0. 157 - 2. 51 g .L^- 1 （ r = 0. 999 6）, the LOD was 39. 3 mg . L ^-1 ,the average recovery of inositol for injection was 99% with RSD of 1.1%. The active ingredient inositol was successfully separated from the other substances. CONCLUSION The method is simple, rapid, accurate and it provides a new reliable means for the quality control of inositol.