摘要
Touchdown PCR扩增溶藻弧菌HY9901 AcrA基因部分序列,得一460 bp片段,再以反向PCR和巢式PCR联合扩增其侧翼序列,拼接得一由1101 nt组成,共编码366 aa的完整基因。该基因演绎的氨基酸序列与几种弧菌的同源性都比较高,与创伤弧菌YJ016、副溶血弧菌RIMD 2210633、灿烂弧菌12B01、霍乱弧菌O1 N16961同源性分别为76%、73%、71%和70%。
A 460 bp internal fragment of the AcrA gene from Vibrio alginolyticus strain HY9901 was amplified by PCR with designed primers and the unknown flanking sequence of 5 ′- and 3′- ends of the AcrA gene was finally characterized by inverse PCR and nested PCR. Sequence analysis showed the AcrA gene contained 1101 bp ORF encoding 366 amino acids and the deduced amino acid sequence of the precursor from Vibrio alginolyticus strain HY9901 showed significant homology with the putative protein of other Vibrio species. The AcrA shows 76%, 73%, 71% and 70% homology with V. vulnificus strain YJ016, V. parahaemolyticus strain RIMD 2210633, V. splendidus strain 12B01 and V. cholerae O1 biovar eltor str. N 16961 respectively.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第1期67-72,共6页
Microbiology China
基金
Guangdong Natural Science Foundation(No.011812)
Guangdong Municipal Science and Technology Project (No.A3050302)
