摘要
获得高质量的土壤总DNA是土壤细菌生态学的关键步骤之一。实验通过综合应用两个试剂盒(Soilmaster kit和DNA IQ^(TM)系统)的优点进行土壤样品总DNA的提取,结果证明该方法是一种快速、有效、灵敏、稳定的土壤DNA提取方法。另外尝试将16S rDNA序列和T-RFLP(Terminal restriction fragment length polymorphism)技术引入土壤细菌DNA群落多样性的研究中,证明T-RFLP是一种有力的土壤细菌多样性分析工具。
Obtaining soil bacterial DNA of good quality is a key step in soil bacterial ecology study. A quick, efficient, sensitive and stably method of DNA extraction from soil were established by combining strongpoints of two kits （ Soilmaster kit and DNA IQ^TM kit）. In addition, the 16S rDNA gene and T-RFLP （Terminal restriction fragment length polymorphism） were used in the analysis of soil bacterial community diversity and the result show that T-RFLP is a powerful tool for bacterial community study.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第1期131-136,共6页
Microbiology China
基金
公安部重点研究项目(No.20038221401)
