摘要
采用双层平板法应用于嗜盐古菌铁载体的原位检测。双层平板的上层为不添加铁离子的嗜盐古菌培养基,嗜盐古菌可在其上生长,在缺铁胁迫下可向外界分泌铁载体;下层为含有CAS检测液用于铁载体检测的琼脂。当上层平板生长的嗜盐古菌分泌的铁载体透过培养基渗透到下层检测琼脂后,即可在下层检测平板上产生明显的特征性的铁载体螯合晕圈,表明双层平板法在嗜盐古菌的铁载体检测中确实可行,且较原有的嗜盐古菌铁载体检测方法简便、直接。
A two-layer plate was developed for in situ detection of siderophore from halophilic archaea. Halophilic archaea could grow on the upper nutrient layer without addition of iron and excrete siderophore under iron-limited stress. The lower layer was the detection agar, which contained universal blue-coloured ferric-CAS complex. The presence of the siderophore was indicated by the decolorization of the blue complex, resulting in a yellow-orange halo around colonies growing on the upper nutrient layer, when the excreted siderophore penetrated from the upper nutrient layer into the lower detection agar. The results showed that the two-layer plate method was applicable to the detection of siderophore from halophilic archaea, which was more convenient and definite than former approach for halophilic archaea siderophore detection.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第1期142-144,共3页
Microbiology China
基金
红河学院硕
博士科研启动基金项目(No.XSZ05024)
关键词
双层平板
嗜盐古菌
铁载体
Two-layer detection plate, Siderophores, Halophilic archaea