摘要
焦磷酸法最早是应用在检测DNA甲基化和SNP位点分析的一项技术,在2005年底,此技术被用来进行基因组序列的测定,已经成为下一代高通量测序中最成熟的一种技术。本篇文章着重介绍了采用焦磷酸测序法的罗氏公司最新一代高通量基因组测序系统GS FLX的技术原理,操作过程和广泛的应用范围。
Pyrosequencing technology is famous for its application in detection of SNP site and DNA methylation. At the end of 2005, pyrosequencing technology was used to sequence the whole genome and it has become the most developed next generation high-throughput sequencing technonlogy. In this article, we introduce the theory, operation process and wide application field of the second generation genome sequencer FLX system provided by Roche .
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第1期149-151,共3页
Microbiology China
关键词
GS
FLX
焦磷酸测序
高通量
读长
应用
GS FLX, Pyrosequencing, High throughput, Read length, Application