内生枯草芽孢杆菌E1R-J发酵条件的优化

Optimizing of Fermentation Conditions of Endophytic Bacteria E1R-J

细菌E1R-J是一株对小麦全蚀病、小麦白粉病和小麦条锈病有拮抗作用的小麦内生芽孢杆菌，笔者对其产菌的发酵培养条件进行了初步研究。发酵培养基为酵母粉0．6％，蛋白胨0．6％，NaCl0．3％，K2HPO40．02％。最佳发酵条件为pH4．5～9．0，菌株都可以生长，转速150r／min，温度34℃，种子液的接种量为2％，通气量是空气与培养基的比例2：5，最佳种子液菌龄是20～34h，作为生防用菌最佳菌龄为48～73h。在以上条件下，细菌的发酵产量最大，细菌浓度为5．2×10^7cfu／mL左右。

The endophytic bacteria EIR-J is isolated from the root of winter wheat, which has the better effect to take-all, powdery mildew and rust of wheat. And that the optimizing of the fermentation conditions showed that the optimum media is that made of peptone 0.6%, yeast exact 0.6%, NaCl 0.3%, K2HPO4 0.02% with initial pH 4.5-9.0, culturing at 34℃, 150 r/min, and inoculation of seeds culture with 2℃, in 100 mL LB of 250 mL flask, the time of seeds culture is 20-34 h, and the time of fermentation for biocontrol is 48-73 h. So the concentration is 5.2 ×10^7cfu/mLo