摘要
建立了合理、可靠的液相色谱-质谱联用测定β-受体激动剂的方法。样品经5％高氯酸溶液提取，反相C18小柱净化，采用Agilent Zorbax Eclipse Plus C18柱分离，以0．1％乙酸水溶液（A）-乙腈（B）为流动相进行等度洗脱。通过液相色谱-质谱以MRM方式检测，测得5种β-受体激动剂的检测限为0．4～1．2μg·kg^-1。对肝脏进行添标回收实验，平均回收率为77．2％～95．6％。
A reasonable and reproducible liquid chromatography-mass spectrometry （LC/ MS） for detecting β-agonists was developed. The sample was extracted with 5 % perchloricacid, centrifuged by a solid-phase extraction procedure with LC-18 cartridges. The products were separated on Agilent Zorbax Eclipse Plus C18 column with a isocratic elution of 0.1% acetate acid and acetonitrile. An electrospray ionization （ESI） source was operated in positive ion mode. The detection limits of five ffagonists are in the range of 0.4-1.2μg · kg^-1. The experimental results of spiked liver indicate that the average recoveries range from 77.2% to 95.6%.
出处
《质谱学报》
EI
CAS
CSCD
2008年第1期10-12,17,共4页
Journal of Chinese Mass Spectrometry Society
基金
国家"十五"重点科技攻关(No.2002BA906A28-1B)资助
