三维荧光光谱结合二阶校正算法测定人体血浆和厚朴药材中的厚朴酚及和厚朴酚

Determination of Honokiol and Magnolol in Both Human Plasma and Complex Magnoliae Officinalis by Second-order Calibration Coupled to Three-dimensional Excitation-emission Fluorescence Spectra

利用三维荧光光谱与化学计量学二阶校正算法相结合,直接测定人体血浆中和厚朴药材中的厚朴酚及和厚朴酚.采用平行因子分析(PARAFAC)算法解析所得两种物质的回收率分别为(99.5±2.6)%和(90.2±1.8)%.采用交替三线性分解(ATLD)算法解析,当组分数N取3时,回收率分别为(104.2±3.2)%和(98.7±4.0)%;当N取4时,回收率分别为(102.7±2.9)%和(99.0±4.6)%.同时用该方法对厚朴药材中的厚朴酚及和厚朴酚进行快速定量测定,结果令人满意.实验结果表明,此法可用于复杂试样中未知干扰共存下厚朴酚及和厚朴酚的同时测定.

A simple method, second-order calibration coupled to three-dimensional excitation-emission fluorescence spectroscopy, was described for the direct determination of honokiol and magnolol in human plasma and complicated magnoliae officinalis. When the component number（N） was set to 3, the obtained recoveries of honokiol and magnolol in human plasma by using the parallel factor analysis （ PARAFAC ） were （ 99.5 ± 2.6） % and （90. 2 ± 1.8 ） %, respectively. As the alternating trilinear decomposition （ATLD） was utilized, the recoveries of honokiol and magnolol were （104.2 ± 3.2 ）% and （98.7 ± 4.0 ）% for N = 3, （102.7 ± 2.9） % and （99.0 4.6 ）% for N = 4, respectively. And the method was used to determine honokiol and magnolol in complex magnoliae officinalis, the obtained results were also satisfactory. It was shown that the proposed method can be used to determine directly honokiol and magnolol, even in the presence of unknown interferences, with a simple pretreatment procedure.