用大孔明胶微载体培养Vero细胞

Culture of Vero Cells on Gelatin based Macroporous Microcarriers

用自制大孔明胶微载体与实心微载体ＣＴ－３在转瓶中培养Ｖｅｒｏ细胞，在培养后期许多细胞从ＣＴ－３上脱落下来，细胞密度降低，而大孔微载体上的细胞密度一直保持在较高水平（８×１０８ｃｅｌｓ／Ｌ以上），表明大孔微载体能够延长细胞活性期。用大孔明胶微载体在反应器中培养Ｖｅｒｏ细胞，最大活细胞密度达５．６×１０９ｃｅｌｓ／Ｌ。

With solid CT 3 microcarriers as a control, Vero cells on gelatin based macroporous microcarriers were cultivated in Wheaton spinner bottles. During first three days of cultivation, cell density on porous carriers was lower than that on CT 3. But at the late stage of cultivation, cell density on porous carriers kept a high level (above 8×10 8 cells/L), whereas cells on CT 3 began to detach. When cells were cultured in a 1.5L CelliGen bioreactor using gelatin based macroporous microcarriers, the viable cell density reached a maximum of 5.6×10 9cells/L.