用微量热法研究漆酶和3,4-二羟基苯甲醛的反应

Study on the Reaction Between Laccase and 3,4 Dihydroxybenzaldehyde by Microcalorimetry

用微量热法研究漆酶和３，４－二羟基苯甲醛的反应望天志＊吴鼎泉万洪文＊＊屈松生（武汉大学化学系，武汉４３００７２，＊＊华中师范大学化学系武汉）关键词漆树漆酶，微量热法，３，４－二羟基苯甲醛１９９６－０８－０８收稿，１９９６－１０－１７修回国家自然科学基...

Thermokinetic properties of the reaction between laccase and 3,4 dihydroxybenzaldehyde have been studied with a microcalorimetry system. The molar reaction enthalpy(Δ r H m), michaelis constant( K m), rate constant( k 2), binding energy(Δ G 0), activation gibbis free energy(Δ G =) and laccase activity( E A) have been determined. The result has been discussed by the transition state theory.